DR1 protein (His tag) (80R-1431)
Purified recombinant Human DR1 protein
|Synonyms||TBP-binding, Down-regulator of transcription 1 protein, DR-1, DR1, NC2-BETA protein, DR 1, DR-1 protein, DR 1 protein, NC2 protein|
Coomassie Blue stained SDS-PAGE of DR1 protein (His tag) (80R-1431)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-176 amino acids: MGSSHHHHHH SSGLVPRGSH MASSSGNDDD LTIPRAAINK MIKETLPNVR VANDARELVV NCCTEFIHLI SSEANEICNK SEKKTISPEH VIQALESLGF GSYISEVKEV LQECKTVALK RRKASSRLEN LGIPEEELLR QQQELFAKAR QQQAELAQQE WLQMQQAAQQ AQLAAASASA SNQAGSSQDE EDDDDI|
|Purity||> 85% pure|
|Molecular Weight||21.6 kDa(196aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl Buffer, pH 8.0, containing 100mM NaCl, 0.1mM PMSF and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||DR1, also known as NC2-beta, is a TBP (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. By selectively repressing polymerases II and III, DR1 may shift the physiological balance of transcriptional output in favor of polymerase I. Recombinant human DR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography|
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