DsbG protein (30R-1281)

Purified recombinant E.coli DsbG protein

Synonyms ybdP protein, Thiol:disulfide interchange protein dsbG ECK0598 protein, Periplasmic disulfide isomerase/thiol disulphide oxidase protein, , JW0597 protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of DsbG protein (30R-1281)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 18-248 amino acids: MEELPAPVKA IEKQGITIIK TFDAPGGMKG YLGKYQDMGV TIYLTPDGKH AISGYMYNEK GENLSNTLIE KEIYAPAGRE MWQRMEQSHW LLDGKKDAPV IVYVFADPFC PYCKQFWQQA RPWVDSGKVQ LRTLLVGVIK PESPATAAAI LASKDPAKTW QQYEASGGKL KLNVPANVST EQMKVLSDNE KLMDDLGANV TPAIYYMSKE NTLQQAVGLP DQKTLNIIMG NK
Expression System E.coli
Purity > 95% pure
Molecular Weight 25.8 kDa(232 aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 2mM EDTA, and 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Dsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. So DsbG was shown to interact with refolding intermediates of chemically denatured citrate synthase and prevents their aggregation in vitro. In addition to sharing sequence homology with the thiol disulfide exchange protein DsbC, DsbG likewise was shown to form a stable periplasmic dimer and it displays an equilibrium constant with glutathione comparable with DsbA and DsbC. DsbG was found to be expressed at approximately 25% the level of DsbC. Recombinant DsbG was expressed in E. coli and purified by conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of DsbG protein (30R-1281) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 100 ug
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