DUSP21 protein (His tag) (80R-2135)

Recombinant human DUSP21 protein (His tag)

Synonyms DUSP-21, DUSP-21 protein, DUSP 21 protein, DUSP 21, Dual specificity protein phosphatase 21 protein, DUSP21
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of DUSP21 protein (His tag) (80R-2135)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-190 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMTASAS SFSSSQGVQQ PSIYSFSQIT RSLFLSNGVA ANDKLLLSSN RITAIVNASV EVVNVFFEGI QYIKVPVTDA RDSRLYDFFD PIADLIHTID MRQGRTLLHC MAGVSRSASL CLAYLMKYHS MSLLDAHTWT KSRRPIIRPN NGFWEQLINY EFKLFNNNTV RMINSPVGNI PDIYEKDLRM MISM
Expression System E.coli
Purity > 90% pure
Molecular Weight 24.1 kDa (214aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl
Concentration 0.25 mg/ml

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance DUSP21 (Dual specificity protein phosphatase 21) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases(DUSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. This enzyme can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. It is exclusively expressed in testis where is preferentially dephosphorylates phosphotyrosine residues in MAPK peptides. Recombinant human DUSP21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of DUSP21 protein (His tag) (80R-2135) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $365.00
Size: 50 ug
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