DUSP3 protein (His tag) (80R-1992)

Recombinant human DUSP3 protein (His tag)

Synonyms DUSP-3 protein, DUSP-3, Dual specificity protein phosphatase 3 protein, DUSP3, DUSP 3 protein, VHR protein, DUSP 3
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of DUSP3 protein (His tag) (80R-1992)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-185 amino acids: MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP
Expression System E.coli
Purity > 95% pure
Molecular Weight 22.6 kDa (205aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations DUSP3 protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.
Bioactivity Specific activity: >1,750 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DUSP3 is expressed in both breast and ovarian tissues. DUSP3 Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. Recombinant human DUSP3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of DUSP3 protein (His tag) (80R-1992) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $275.00
Size: 100 ug
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