DUT antibody (70R-1053)

Rabbit polyclonal DUT antibody raised against the C terminal of DUT

Synonyms Polyclonal DUT antibody, Anti-DUT antibody, dUTPase antibody, FLJ20622 antibody, Dutp Pyrophosphatase antibody
Specificity DUT antibody was raised against the C terminal of DUT
Cross Reactivity Human,Mouse,Rat,Dog
Applications IHC, WB
Immunogen DUT antibody was raised using the C terminal of DUT corresponding to a region with amino acids NFGKEKFEVKKGDRIAQLICERIFYPEIEEVQALDDTERGSGGFGSTGKN
Assay Information DUT Blocking Peptide, catalog no. 33R-6682, is also available for use as a blocking control in assays to test for specificity of this DUT antibody

Images

Western Blot analysis using DUT antibody (70R-1053)

DUT antibody (70R-1053) used at 2.5 ug/ml to detect target protein.

Specifications

Host Rabbit
Method of Purification Total IgG Protein A purified
Molecular Weight 19 kDa (MW of target protein)
Form & Buffer Lyophilized powder. Add 100ul distilled water for a 1mg/ml concentration of DUT antibody in PBS
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 2.5 ug/ml; IHC: 4-8 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for short periods. For longer periods of storage, store at -20 deg C. Avoid repeat freeze-thaw cycles.

General Information

Biological Significance DUT is an essential enzyme of nucleotide metabolism. This protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Western Blot analysis using DUT antibody (70R-1053) | DUT antibody (70R-1053) used at 2.5 ug/ml to detect target protein.
  • Immunohistochemical staining using DUT antibody (70R-1053) | DUT antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Myocardial cells (arrows) in Human Heart. Magnification is at 400X

Availability: In stock

Price: $275.00
Size: 100 ug
OR
Shipping
View Our Distributors