E. coli O157 antibody (10C-CR1295M3)

Mouse monoclonal E. coli O157 antibody

Synonyms Monoclonal E. coli O157 antibody, Anti-E. coli O157 antibody, E.coli antibody, Escherichia Coli O157 antibody
Specificity E Coli O157
Cross Reactivity This antibody recognizes the 'O' antigen of E coli serotype O157 Non-reactive with E coli serotypes: O111, O125, O20, O55 & K14
Applications ELISA, IF
Immunogen E. coli O157 antibody was raised in mouse using 'O' antigen of E. coli serotype O157 as the immunogen.

Specifications

Host Mouse
Clone 1516501
Isotype IgG3
Purity > 90% pure
Form & Buffer PBS , pH 7.2, with 0.1% NaN3.
Concentration Batch dependent

Usage & Assay Information

Usage Recommendations ELISA: 1:20-1:200, IF: 1:10-1:50

Storage & Safety

Storage Aliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance E. coli is a Gram-negative rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some, such as serotype O157:H7, can cause serious food poisoning in humans, and are occasionally responsible for product recalls. The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, and by preventing the establishment of pathogenic bacteria within the intestine.

References
Add a Paper

Zhu Fu, Snezna Rogelj, Thomas L. Kieft

Rapid detection of Escherichia coli O157:H7 by immunomagnetic separation and real-time PCR

International Journal of Food Microbiology

Volume: 99 Issue: 1 Page: 47-57 DOI: 10.1016/j.ijfoodmicro.2004.07.013

Daniel R Shelton, Jo Ann S Van Kessela, Marian R Wachtelb, Kenneth T Beltc, Jeffrey S Karns

Evaluation of parameters affecting quantitative detection of Escherichia coli O157 in enriched water samples using immunomagnetic electrochemiluminescence

Journal of Microbiological Methods

Volume: 55 Issue: 3 Page: 717-725 DOI: 10.1016/j.mimet.2003.07.004

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Availability: In stock

Price: $325.00
Size: 100 ug
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