E2F5 antibody (10R-1555)

Mouse monoclonal E2F5 antibody

Synonyms Monoclonal E2F5 antibody, Anti-E2F5 antibody, E2F5, EF5 2 antibody, EF5 2, EF5-2 antibody, EF5-2, E2F transcription factor 5 p130-binding antibody
Specificity Human E2F5
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen E2F5 antibody was raised in mouse using recombinant Human Transcription Factor E2F-5

Images

Immunofluorescent staining using E2F5 antibody (10R-1555)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-E2F5 antibody.

Specifications

Host Mouse
Clone 220D1a
Isotype IgG1
Method of Purification E2F5 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer E2F5 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members.

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Images

  • Immunofluorescent staining using E2F5 antibody (10R-1555) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-E2F5 antibody.
  • Western Blot analysis of immunized recombinant protein using E2F5 antibody (10R-1555)

Availability: In stock

Price: $190.00
Size: 50 ug
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