E2F6 antibody (10R-1292)

Mouse monoclonal E2F6 antibody

Synonyms Monoclonal E2F6 antibody, Anti-E2F6 antibody, EF6-2 antibody, EF6-2, E2F-6 antibody, E2F6, EF6 2 antibody, EF6 2, E2F transcription factor 6 antibody
Specificity Human E2F6
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen E2F6 antibody was raised in mouse using recombinant Human E2F Transcription Factor 6, Transcript Variant A

Images

Immunofluorescent staining using E2F6 antibody (10R-1292)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-E2F6 antibody.

Specifications

Host Mouse
Clone E2F6B9E2
Isotype IgG1
Method of Purification E2F6 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer E2F6 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max.

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Images

  • Immunofluorescent staining using E2F6 antibody (10R-1292) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-E2F6 antibody.
  • Western Blot analysis of immunized recombinant protein using E2F6 antibody (10R-1292)

Availability: In stock

Price: $190.00
Size: 50 ug
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