ECHDC1 protein (His tag) (80R-2166)
Purified recombinant Human ECHDC1 protein (His tag)
|Synonyms||ECHDC1, ECHDC-1, Enoyl CoA hydratase domain containing 1 protein, dJ351K20.2 protein, ECHDC 1 protein, ECHDC-1 protein, ECHDC 1|
Coomassie Blue stained SDS-PAGE of ECHDC1 protein (His tag) (80R-2166)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MGSSHHHHHH SSGLVPRGSH MALKQEMAKS LLKTASLSGR TKLLHQTGLS LYSTSHGFYE EEVKKTLQQF PGGSIDLQKE DNGIGILTLN NPSRMNAFSG VMMLQLLEKV IELENWTEGK GLIVRGAKNT FSSGSDLNAV KSLGTPEDGM AVCMFMQNTL TRFMRLPLIS VALVQGWALG GGAEFTTACD FRLMTPESKI RFVHKEMGII PSWGGTTRLV EIIGSRQALK VLSGALKLDS KNALNIGMVE EVLQSSDETK SLEEAQEWLK QFIQGPPEVI RALKKSVCSG RELYLEEALQ NERDLLGTVW GGPANLEAIA KKGKFNK|
|Purity||> 95% pure|
|Molecular Weight||35.8 kDa|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||ECHDC1 belongs to the enoyl-CoA hydratase/isomerase family. Enoyl-CoA hydratase is an enzyme that hydrates the double bond between the second and third carbons on acyl-CoA. This enzyme is essential to metabolizing fatty acids to produce both acetyl CoA and energy. Recombinant human ECHDC1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
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