EEA1 antibody (10R-1579)

Mouse monoclonal EEA1 antibody

Synonyms Monoclonal EEA1 antibody, Anti-EEA1 antibody, EEA 1 antibody, EEA-1 antibody, EEA1, ZFYVE2 antibody, EEA 1, Early Endosome Antigen 1 antibody, EEA-1
Specificity Human EEA1
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen EEA1 antibody was raised in mouse using recombinant Human Early Endosome Antigen 1, 162Kd (Eea1)

Images

Immunofluorescent staining using EEA1 antibody (10R-1579)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-EEA1 antibody.

Specifications

Host Mouse
Clone 2306C5a
Isotype IgG1
Method of Purification EEA1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer EEA1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance This gene binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

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Images

  • Immunofluorescent staining using EEA1 antibody (10R-1579) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-EEA1 antibody.
  • Western Blot analysis of immunized recombinant protein using EEA1 antibody (10R-1579)

Availability: In stock

Price: $190.00
Size: 50 ug
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