EFNB2 protein (His tag) (80R-1976)

Recombinant human EFNB2 protein (His tag)

Synonyms Ephrin-B2 protein, Htk-L protein, EFNB2, EPLG5 protein, EFNB-2 protein, EFNB-2, LERK5 protein, HTKL protein, EFNB 2 protein, EFNB 2
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of EFNB2 protein (His tag) (80R-1976)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 28-229 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMIVLEP IYWNSSNSKF LPGQGLVLYP QIGDKLDIIC PKVDSKTVGQ YEYYKVYMVD KDQADRCTIK KENTPLLNCA KPDQDIKFTI KFQEFSPNLW GLEFQKNKDY YIISTSNGSL EGLDNQEGGV CQTRAMKILM KVGQDASSAG STRNKDPTRR PELEAGTNGR SSTTSPFVKP NPGSSTDGNS AGHSGNNILG SEVALFA
Expression System E.coli
Purity > 85% pure
Molecular Weight 24.9 kDa (227aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200mM NaCl, 1mM EDTA
Concentration 0.5 mg/ml

Usage & Assay Information

Usage Recommendations EFNB2 protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance Ephrin-B2 is a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. It binds to the EPHB4 and EPHA3 receptors. Recombinant human EFNB2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

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Images

  • Coomassie Blue stained SDS-PAGE of EFNB2 protein (His tag) (80R-1976) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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