EGF protein (30R-AE003)

Purified recombinant Human EGF protein

Synonyms Epidermal Growth Factor protein, Urogastrone protein, URG protein
Species Human
Protein Type Recombinant
Applications User optimized
Assay Information EGF quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. 2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard.

Specifications

Residues Epidermal Growth Factor protein containing 53 amino acids.
Expression System E.coli
Purity > 98% pure
Form & Buffer Supplied as a lyophilized powder. Reconstitute the lypholized rHuEGF in sterile water not less than 100ug/ml.

Usage & Assay Information

Bioactivity The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

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Availability: In stock

Price: $325.00
Size: 1 mg
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