EGF protein (His tag) (80R-2272)
Purified recombinant Mouse EGF Protein (His tag)
|Synonyms||Urogastrone protein, Epidermal Growth Factor protein, URG protein|
Coomassie Blue stained SDS-PAGE of EGF protein (His tag) (80R-2272)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MGSSHHHHHH SSGLVPRGSH MGSMNSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR|
|Purity||> 90% pure|
|Molecular Weight||8.6 kDa|
|Form & Buffer||Supplied in liquid form in 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl|
Storage & Safety
|Storage||Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.|
|Biological Significance||Recombinant mouse epidermal growth factor is a 8.6 kDa globular protein containing 77 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant mouse EGF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.|
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