EGFR antibody (10R-3131)

Mouse monoclonal EGFR antibody

Synonyms Monoclonal EGFR antibody, anti-EGFR antibody, HER1 antibody, PIG61 antibody, Proto-oncogene c-ErbB-1 antibody, Cell growth inhibiting protein 40 antibody, epidermal growth factor receptor antibody, Oncogene ERBB antibody, Avian erythroblastic leukemia viral (verbb) oncogene homolog antibody, ERBB antibody, Urogastrone antibody, Epidermal growth factor receptor antibody, ERBB1 antibody, Cell proliferation inducing protein 61 antibody
Specificity Human
Applications ELISA, ICC, WB
Immunogen EGFR antibody was raised in mouse using recombinant human EGFR (424-605aa) purified from E. coli as the immunogen.

Images

Immunofluorescent staining using EGFR antibody (10R-3131)

Immunofluorescenitrocellulosee of HaCaT cells stained with Hoechst 3342 (Blue) or phalloidin-TRITC for nucleus or actin staining, respectably. monoclonal anti-human EGFR antibody (1:250) was stained with alexa 488 (green).

Specifications

Host Mouse
Clone AT6E3
Isotype IgG2b kappa
Method of Purification EGFR antibody was purified by protein G affinity chromatography.
Form & Buffer Supplied in liquid form in Phosphate-Buffered Saline, pH 7.4 with 0.1% Sodium Azide.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations ELISA, ICC, WB, ELISA: 1:250, ICC: 1:250, WB: 1:250

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Epidermal growth factor receptor (EGFR) is a 170 kDa membrane protein first identified as a binding partner of EGF. Also, EGFR phosphorylated itself (autophosphorylation) in response to EGF treatment. The overexpression of EGFR in various epithelial tumors was widely reported and it has been substantiated that deregulation of EGFR itself and its signaling pathways an important role in human cancers.

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Images

  • Immunofluorescent staining using EGFR antibody (10R-3131) | Immunofluorescenitrocellulosee of HaCaT cells stained with Hoechst 3342 (Blue) or phalloidin-TRITC for nucleus or actin staining, respectably.  monoclonal anti-human EGFR antibody (1:250) was stained with alexa 488 (green).
  • Western Blot analysis of EGFR antibody (10R-3131) | The extracts of HaCaT were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFR (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: $265.00
Size: 100 ul
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