EGFR antibody (20-ES04)

Sheep polyclonal EGFR antibody

Synonyms Polyclonal EGFR antibody, Anti-EGFR antibody, v-erb-b homolog antibody, Epidermal Growth Factor antibodyEgf receptor antibody, egf-r antibody, panitumumab antibody, egfr antibody, epidermal growth factor receptor antibody, erbb1 antibody
Specificity Human
Cross Reactivity Mouse, rat, turkey
Applications IC, IHC-P, IP, WB
Immunogen EGFR antibody was raised in sheep using a synthetic 20 amino acid peptide of human EGFr representing a portion of the internal domain proximal to a phosphorylation region as the immunogen.


Immunofluorescent staining using EGFR antibody (20-ES04)

Immunofluorescently labelled cells with sheep anti-EGFR.


Host Sheep
Form & Buffer Protein G purified and supplied in PBS, pH 7.2.
Concentration Typically 1-5 mg/ml

Usage & Assay Information

Usage Recommendations WB: 10-50 ug/ml, IC: 25-100 ug/ml, IHC-P: 5 ug/ml, IP: 10-12 ug/ml, WB: 10-50 ug/ml

Storage & Safety

Storage Aliquot and store at -20 deg C. Avoid repeated Freeze/Thaw cycles.

General Information

Biological Significance The epidermal growth factor receptor (EGFR) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGF alpha).

Add a Paper

Alice Bjerregaard Larsen, Marie-Thérése Stockhausen, Hans Skovgaard Poulsen

Cell adhesion and EGFR activation regulate EphA2 expression in cancer

Cellular Signalling

Volume: 22 Issue: 4 Page: 636-644 DOI: 10.1016/j.cellsig.2009.11.018

Morten P Oksvolda, Henrik S Huitfeldtb, Wallace Y Langdon

Identification of 14-3-3 zeta as an EGF receptor interacting protein

FEBS Letters

Volume: 569 Issue: 1-3 Page: 207-210 DOI: 10.1016/j.febslet.2004.05.068

Vibeke Bertelsena, Kamilla Breena, Kirsten Sandvigb, Espen Stanga, Inger Helene Madshus

The Cbl-interacting protein TULA inhibits dynamin-dependent endocytosis

Experimental Cell Research

Volume: 313 Issue: 8 Page: 1696-1709 DOI: 10.1016/j.yexcr.2007.02.017

Lene Melsæther Grøvdal, Lene E. Johannessen, Marianne Skeie Rødland, Inger Helene Madshus, Espen Stang

Dysregulation of Ack1 inhibits down-regulation of the EGF receptor

Experimental Cell Research

Volume: 314 Issue: 6 Page: 1292-1300 DOI: 10.1016/j.yexcr.2007.12.017

Marie-Thérése Stockhausen, Helle Broholm, Mette Villingshøj, Maria Kirchhoff, Tommy Gerdes, Karina Kristoffersen, Michael Kosteljanetz, Mogens Spang-Thomsen, Hans Skovgaard Poulsen

Maintenance of EGFR and EGFRvIII expressions in an in vivo and in vitro model of human glioblastoma multiforme

Experimental Cell Research

Volume: 317 Issue: 11 Page: 1513-1526 DOI: 10.1016/j.yexcr.2011.04.001

Sissel Beate Rønninga, Nina Marie Pedersenb, Inger Helene Madshusa, Espen Stang

CIN85 regulates ubiquitination and degradative endosomal sorting of the EGF receptor

Experimental Cell Research

Volume: 317 Issue: 13 Page: 1804-1816 DOI: 10.1016/j.yexcr.2011.05.016

Matsui T, Noguchi K, Fukuda M.

Dennd3 functions as a guanine nucleotide exchange factor for small GTPase Rab12 in mouse embryonic fibroblasts

J Biol Chem

Volume: 289 Issue: 20 Page: 13986-95 PMID: 24719330 DOI: 10.1074/jbc.M113.546689


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  • Immunofluorescent staining using EGFR antibody (20-ES04) | Immunofluorescently labelled cells with sheep anti-EGFR.

Availability: In stock

Price: $324.50
Size: 200 ug
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