EGLN3 protein (His tag) (80R-2348)
Purified recombinant Human EGLN3 Protein (His tag)
|Synonyms||EGLN-3, EGLN 3, EGLN-3 protein, HIFPH3 protein, EGLN 3 protein, Egl nine homolog 3 protein, EGLN3, PHD3 protein|
Coomassie Blue stained SDS-PAGE of EGLN3 protein (His tag) (80R-2348)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MGSSHHHHHH SSGLVPRGSH MGSHMPLGHI MRLDLEKIAL EYIVPCLHEV GFCYLDNFLG EVVGDCVLER VKQLHCTGAL RDGQLAGPRA GVSKRHLRGD QITWIGGNEE GCEAISFLLS LIDRLVLYCG SRLGKYYVKE RSKAMVACYP GNGTGYVRHV DNPNGDGRCI TCIYYLNKNW DAKLHGGILR IFPEGKSFIA DVEPIFDRLL FFWSDRRNPH EVQPSYATRY AMTVWYFDAE ERAEAKKKFR NLTRKTESAL TED|
|Molecular Weight||29.8 kDa|
|Form & Buffer||Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.3M NaCl, 5mM DTT, 2mM EDTA|
Usage & Assay Information
|Bioactivity||Measured by its ability to induce alkaline phosphatase production using ATDC5 mouse chondrogenic cells. The ED50 for this effect is < 57 ng/ml.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1-2 weeks). Aliquot and store at -20 deg C to -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biohazard Information||< 1.0 EU per 1 ug of protein (determined by LAL method)|
|Biological Significance||EGLN3, a member of the EGLN family of prolyl hydroxylases, has been shown to catalyze hydroxylation of the a subunit of hypoxia-inducible factor-a, which targets hypoxia-inducible factor-a for ubiquitination by a ubiquitin ligase complex containing the von Hippel-Lindau (VHL) tumor suppressor. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM2 in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Recombinant human EGLN3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.|
Sorry, but there are no references currently for this product.
Sorry there are currently no reviews for this product