EHMT1 antibody (10R-1468)

Mouse monoclonal EHMT1 antibody

Synonyms Monoclonal EHMT1 antibody, Anti-EHMT1 antibody, EHMT-1, EHMT-1 antibody, EHMT 1 antibody, bA188C12.1 antibody, FP13812 antibody, DKFZp667M072 antibody, RP11-188C12.1 antibody, FLJ12879 antibody, EHMT1, GLP antibody, KIAA1876 antibody, EHMT 1, Eu-HMTase1 antibody, EUHMTASE1 antibody
Specificity Human EHMT1
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen EHMT1 antibody was raised in mouse using recombinant Homosapienseuchromatichistone-Lysinen-Methyltransferase1

Images

Immunofluorescent staining using EHMT1 antibody (10R-1468)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-EHMT1 antibody.

Specifications

Host Mouse
Clone 2312C2a
Isotype IgG1
Method of Purification EHMT1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer EHMT1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Histone methyltransferase. Methylates 'Lys-9' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle.

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Images

  • Immunofluorescent staining using EHMT1 antibody (10R-1468) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-EHMT1 antibody.
  • Western Blot analysis of immunized recombinant protein using EHMT1 antibody (10R-1468) | Left: EHMT1 staining. Right: Coomassie Blue staining of immunized recombinant protein.

Availability: In stock

Price: $190.00
Size: 50 ug
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