EIF2S1 antibody (10R-1784)
Mouse monoclonal EIF2S1 antibody
|Synonyms||Monoclonal EIF2S1 antibody, Anti-EIF2S1 antibody, EIF 2 alpha antibody, EIF-2A antibody, EIF2 alpha antibody, EIF2A antibody, EIFS1-2, EIF2 antibody, EIF-2 antibody, EIFS1-2 antibody, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody, Eukaryotic translation initiation factor 2 subunit 1 alpha antibody, # Eukaryotic translation initiation factor 2 subunit alpha antibody, EIFS1 2, EIFS1 2 antibody, EIF 2 antibody, Eukaryotic translation initiation factor 2 subunit 1 antibody, EIF 2A antibody, EIF-2alpha antibody, EIF 2alpha antibody|
|Immunogen||EIF2S1 antibody was raised in mouse using recombinant human EIF2S1 (1-315aa) purified from E. coli as the immunogen.|
Western Blot analysis of EIF2S1 antibody (10R-1784)
The extracts of HeLa (50 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF2S1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
|Source||Anti-human EIF2S1 antibody, clone 5E10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human EIF2S1 protein.|
|Method of Purification||By protein-G affinity chromatography|
|Form & Buffer||Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide|
Usage & Assay Information
|Usage Recommendations||Each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000.|
Storage & Safety
|Storage||Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.|
|Biological Significance||Eukaryotic translation initiation factor 2, subunit 1 alpha (EIF2S1) is plays a central role in initiating translation, and provides for a rate-limiting step in protein synthesis. The phosphorylation of alpha-subunit of EIF2S1 effectively prevents formation of the EIF2S1/GTP/Met-tRNAi complex and inhibits global protein synthesis.|
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