EIF2S1 protein (His tag) (80R-1344)
Purified recombinant Human EIF2S1 protein
|Synonyms||EIF 2alpha protein, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa protein, Eukaryotic translation initiation factor 2 subunit 1 alpha protein, EIF2A protein, EIF-2A protein, EIF-2 protein, Eukaryotic translation initiation factor 2 subunit 1 EIF 2 alpha protein, EIF2S1, EIFS1-2, EIF2 alpha protein, EIF 2 protein, EIF-2alpha protein, EIFS1 2 protein, EIF 2A protein, # Eukaryotic translation initiation factor 2 subunit alpha., EIF2 protein, EIFS1-2 protein, EIFS1 2|
Coomassie Blue stained SDS-PAGE of EIF2S1 protein (His tag) (80R-1344)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-315 amino acids: MGSSHHHHHH SSGLVPRGSH MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRRV SPEEAIKCED KFTKSKTVYS ILRHVAEVLE YTKDEQLESL FQRTAWVFDD KYKRPGYGAY DAFKHAVSDP SILDSLDLNE DEREVLINNI NRRLTPQAVK IRADIEVACY GYEGIDAVKE ALRAGLNCST ENMPIKINLI APPRYVMTTT TLERTEGLSV LSQAMAVIKE KIEEKRGVFN VQMEPKVVTD TDETELARQM ERLERENAEV DGDDDAEEME AKAED|
|Purity||> 85% pure|
|Molecular Weight||38.2 kDa (335aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||EIF2S1, also known as eIF2 alpha, is the alpha subunit of the translation initiation factor eIF2 complex which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. The phosphorylation state of eIF2S1 controls the rate of tRNA translation. When eIF2 alpha is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2S1 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. Recombinant human EIF2S1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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