EIF4E protein (His tag) (80R-1425)

Purified recombinant Human EIF4E protein

Synonyms EIF4E, EIFE-4, Eukaryotic translation initiation factor 4E, EIF4EL1 protein, EIF4F protein, EIFE-4 protein, EIFE 4, CBP protein, EIFE 4 protein, EIF4E1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of EIF4E protein (His tag) (80R-1425)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-217 amino acids: MGSSHHHHHH SSGLVPRGSH MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV
Expression System E.coli
Purity > 85% pure
Molecular Weight 27.2 kDa(237aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl Buffer, pH 8.0, containing 10% glycerol.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance EIF4E, a member of eukaryotic initiation factor 4 families, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Recombinant human EIF4E protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of EIF4E protein (His tag) (80R-1425) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $395.00
Size: 50 ug
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