EIF4ENIF1 antibody (10R-1462)

Mouse monoclonal EIF4ENIF1 antibody

Synonyms Monoclonal EIF4ENIF1 antibody, Anti-EIF4ENIF1 antibody, Eukaryotic translation initiation factor 4E nuclear import factor 1 antibody, FLJ21601 antibody, EIFENIF1-4, EIFENIF1 4 antibody, EIFENIF1 4, EIF4ENIF1, Clast4 antibody, EIFENIF1-4 antibody, 4E-T antibody
Specificity Human EIF4ENIF1
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen EIF4ENIF1 antibody was raised in mouse using recombinant Human Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (Eif4Enif1)

Images

Immunofluorescent staining using EIF4ENIF1 antibody (10R-1462)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-EIF4ENIF1 antibody.

Specifications

Host Mouse
Clone 2314C3a
Isotype IgG1
Method of Purification EIF4ENIF1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer EIF4ENIF1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic;its own nuclear import is regulated by a nuclear localization signal and nuclear export signals.

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Images

  • Immunofluorescent staining using EIF4ENIF1 antibody (10R-1462) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-EIF4ENIF1 antibody.
  • Western Blot analysis of immunized recombinant protein using EIF4ENIF1 antibody (10R-1462)

Availability: In stock

Price: $190.00
Size: 50 ug
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