Elongin B protein (30R-1236)

Purified recombinant Human Elongin B protein

Synonyms Elongin B protein, Transcription elongation factor B polypeptide 2 protein, Elongin 18 kDa subunit protein, SIII p18 protein, TCEB 2 protein, EloB protein, EloB protein, RNA polymerase II transcription factor SIII subunit B protein, Transcription elongation factor B (SIII) polypeptide 2., TCEB2 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of Elongin B protein (30R-1236)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-118 amino acids: MDVFLMIRRH KTTIFTDAKE SSTVFELKRI VEGILKRPPD EQRLYKDDQL LDDGKTLGEC GFTSQTARPQ APATVGLAFR ADDTFEALCI EPFSSPPELP DVMKPQDSGS SANEQAVQ
Expression System E.coli
Purity > 95% pure
Molecular Weight 13.1 kDa (118 aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 0.1mM PMSF, and 20% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Elongin B , also known as TCEB2, is a subunit of the transcription factor B (SIII) complex. The SIII complex is a heterotrimer consisting of a transcriptionally active subunit (A) and two regulatory subunits (B and C). It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. The von Hippel-Lindau tumor suppressor protein (pVHL) binds to elongins B and C, and thereby inhibits transcription elongation. Recombinant TCEB2 was expressed as insoluble protein aggregate in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

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Images

  • Coomassie Blue stained SDS-PAGE of Elongin B protein (30R-1236) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $180.00
Size: 100 ug
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