EphB2 antibody (10R-2137)

Mouse monoclonal EphB2 antibody

Synonyms Monoclonal EphB2 antibody, Anti-EphB2 antibody, EPH receptor B2 antibody, Ephrin receptor B2 antibody, EphB2, EphB 2, EphB-2, EphB 2 antibody, EphB-2 antibody
Cross Reactivity Human
Applications ELISA, IF, WB
Immunogen EphB2 antibody was raised in Mouse using a purified recombinant fragment of EphB2(aa17-200) expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using EphB2 antibody (10R-2137) (EphB2 antibody)

Immunofluorescence analysis of Hela (left) and HepG2 (right) cells using EphB2 antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Specifications

Host Mouse
Clone 2D12C6
Isotype IgG2b
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family.

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Images

  • Immunofluorescent staining using Western Blot analysis using EphB2 antibody (10R-2137) (EphB2 antibody) | Immunofluorescence analysis of Hela (left) and HepG2 (right) cells using EphB2 antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Western Blot analysis using EphB2 antibody (10R-2137) | Western Blot showing EphB2 antibody used against truncated EphB2 recombinant protein (1) and extracellular EphB2 (aa19-476)-hIgGFc transfected CHO-K1 cell lysate (2).

Availability: In stock

Price: $375.00
Size: 100 ul
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