EPHB4 antibody (10R-1844)
Mouse monoclonal EPHB4 antibody
|Synonyms||Monoclonal EPHB4 antibody, Anti-EPHB4 antibody, EPHB 4 antibody, EPHB-4, EPHB4, Ephrin receptor B4 antibody, EPHB-4 antibody, EPHB 4, EPH receptor B4 antibody|
|Applications||ELISA, IHC, WB|
|Immunogen||EPHB4 antibody was raised in Mouse using a purified recombinant fragment of EPHB4 expressed in E. coli as the immunogen.|
Immunohistochemical staining using EPHB4 antibody (10R-1844)
Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma (left) and breast carcinoma (right) tissue, showing membrane and cytoplasmic (pancreas carcinoma) localization, membrane (breast carcinoma) localization using EphB4 antibody with DAB staining.
|Form & Buffer||Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family.|
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