EPHB4 antibody (10R-1844)

Mouse monoclonal EPHB4 antibody

Synonyms Monoclonal EPHB4 antibody, Anti-EPHB4 antibody, EPHB 4 antibody, EPHB-4, EPHB4, Ephrin receptor B4 antibody, EPHB-4 antibody, EPHB 4, EPH receptor B4 antibody
Cross Reactivity Human
Applications ELISA, IHC, WB
Immunogen EPHB4 antibody was raised in Mouse using a purified recombinant fragment of EPHB4 expressed in E. coli as the immunogen.

Images

Immunohistochemical staining using EPHB4 antibody (10R-1844)

Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma (left) and breast carcinoma (right) tissue, showing membrane and cytoplasmic (pancreas carcinoma) localization, membrane (breast carcinoma) localization using EphB4 antibody with DAB staining.

Specifications

Host Mouse
Clone 5B8F7
Isotype IgG2a
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family.

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Images

  • Immunohistochemical staining using EPHB4 antibody (10R-1844) | Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma (left) and breast carcinoma (right) tissue, showing membrane and cytoplasmic (pancreas carcinoma) localization, membrane (breast carcinoma) localization using EphB4 antibody with DAB staining.
  • Western Blot analysis using EPHB4 antibody (10R-1844) | Western Blot showing EphB4 antibody used against truncated EphB4 recombinant protein.

Availability: In stock

Price: $195.00
Size: 100 ul
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