EPHB4 antibody (10R-2059)

Mouse monoclonal EPHB4 antibody

Synonyms Monoclonal EPHB4 antibody, Anti-EPHB4 antibody, Myk1 antibody, EPHB-4 antibody, EPHB4, Htk antibody, EPHB 4 antibody, EPH receptor B4 antibody, EPHB 4, Tyro11 antibody, EPHB-4
Cross Reactivity Human
Applications ELISA, IF, WB
Immunogen EPHB4 antibody was raised in Mouse using purified recombinant extracellular fragment of human EPHB4 fused with hIgGFc tag expressed in HEK293 cell line as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using EPHB4 antibody (10R-2059) (EPHB4 antibody)

Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with EphB4-hIgGFc using EphB4 antibody (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.

Specifications

Host Mouse
Clone 5B5
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance EPHB4 is a member of the Eph receptor family, which bind the ephrin ligand family. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The EPHB4 protein binds to ephrin-B2 and plays an essential role in vascular development.

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Images

  • Immunofluorescent staining using Western Blot analysis using EPHB4 antibody (10R-2059) (EPHB4 antibody) | Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with EphB4-hIgGFc using EphB4 antibody (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
  • Western Blot analysis using EPHB4 antibody (10R-2059) | Western Blot showing EphB4 antibody used against extracellular domain of human EphB4 (aa16-539).

Availability: In stock

Price: $345.00
Size: 100 ul
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