ER alpha antibody (10R-1831)

Mouse monoclonal ER alpha antibody

Synonyms Monoclonal ER alpha antibody, Anti-ER alpha antibody, NR3A1 antibody, ERA antibody, Estrogen receptor alpha antibody
Cross Reactivity Human
Applications ELISA, IHC, WB
Immunogen ER alpha antibody was raised in Mouse using a purified recombinant fragment of human ER-alpha (410-592aa) expressed in E. coli as the immunogen.

Images

Immunohistochemical staining using ER alpha antibody (10R-1831)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ER-alpha antibody showing nuclear expression with DAB staining.

Specifications

Host Mouse
Clone 2B7
Isotype IgG1
Form & Buffer Purified antibody supplied in PBS containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The estrogen receptor (ESR) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ESR1 mRNA transcripts, which differ primarily in their 5-prime untranslated regions. The translated receptors show less variability.

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Images

  • Immunohistochemical staining using ER alpha antibody (10R-1831) | Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ER-alpha antibody showing nuclear expression with DAB staining.
  • Western Blot analysis using ER alpha antibody (10R-1831) | Western Blot showing ER-alpha antibody used against human breast carcinoma tissue lysate.

Availability: In stock

Price: $245.00
Size: 100 ul
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