ERK1/2 antibody (10R-2169)

Mouse monoclonal ERK 1/2 antibody

Synonyms Monoclonal ERK 1/2 antibody, Anti-ERK 1/2 antibody, ERK1/2, ERK 1/2 antibody, ERK 1 and 2 antibody, ERK 1 2 antibody, Extracellular signal-regulated kinases 1/2 antibody, ERK 1/2, ERK-1/2 antibody, ERK-1/2
Cross Reactivity Human, Mouse
Applications ELISA, FC, WB
Immunogen ERK 1/2 antibody was raised in Mouse using a purified recombinant fragment of human MAPK1 expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using ERK 1/2 antibody (10R-2169) (ERK 1/2 antibody)

FC analysis of Jurkat cells using p44/42 MAPK antibody (green) and negative control (purple).

Specifications

Host Mouse
Clone 3F8
Isotype IgG2b
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, FC: 1:200-1:400, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The ERKs (MAPK p42/p44) are the prototypic MAPKs and are activated by a variety of mitogenic stimuli as well as differentiation signals. MAPK activation largely requires Ras activation. In Drosophila, the ERK homologue is encoded by the Rolled locus (an activated allele) which has been placed downstream of Ras in the sevenless receptor kinase pathway.

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Images

  • Flow Cytometric assay using Western Blot analysis using ERK 1/2 antibody (10R-2169) (ERK 1/2 antibody) | FC analysis of Jurkat cells using p44/42 MAPK antibody (green) and negative control (purple).
  • Western Blot analysis using ERK 1/2 antibody (10R-2169) | Western Blot showing p44/42 MAPK antibody used against Jurkat (1), Hela (2), A431 (3) and NIH/3T3 (4) cell lysate.
  • Immunohistochemical staining using ERK 1/2 antibody (10R-2169) | Immunohistochemical analysis of paraffin-embedded human Liver tissues using anti-BHMT antibody

Availability: In stock

Price: $325.00
Size: 100 ul
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