ERK2 antibody (10R-2028)

Mouse monoclonal ERK2 antibody

Synonyms Monoclonal ERK2 antibody, Anti-ERK2 antibody, MAPK2 antibody, ERK2, PRKM1 antibody, ERK-2 antibody, ERK 2 antibody, MAPK 2 antibody, p38 antibody, P42MAPK antibody, p41mapk antibody, ERK-2 antibody, Mitogen-activated protein kinase 1 MAP kinase 2 antibody, ERK 2 antibody, ERK 2, ERT1 antibody, p40 antibody, PRKM2 antibody, ERK-2, p41 antibody
Cross Reactivity Human, Mouse, Monkey
Applications ELISA, IF, IHC, WB
Immunogen ERK2 antibody was raised in Mouse using a purified recombinant fragment of human ERK2 expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using ERK2 antibody (10R-2028) (ERK2 antibody)

Confocal immunofluorescence analysis of Eca-109 cells using ERK2 antibody (green).

Specifications

Host Mouse
Clone 4C11
Isotype IgG2a
Form & Buffer Purified antibody supplied in PBS containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance MAPK1 acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Recombinant human MAPK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Immunofluorescent staining using Western Blot analysis using ERK2 antibody (10R-2028) (ERK2 antibody) | Confocal immunofluorescence analysis of Eca-109 cells using ERK2 antibody (green).
  • Western Blot analysis using ERK2 antibody (10R-2028) | Western Blot showing ERK2 antibody used against Hela (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.
  • Immunohistochemical staining using ERK2 antibody (10R-2028) | Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and breast carcinoma (right) showing cytoplasmic localization using ERK2 antibody with DAB staining.

Availability: In stock

Price: $295.00
Size: 100 ul
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