ETV3 antibody (10R-1245)

Mouse monoclonal ETV3 antibody

Synonyms Monoclonal ETV3 antibody, Anti-ETV3 antibody, ETV3, METS antibody, PE1 antibody, PE-1 antibody, ETV-3 antibody, bA110J1.4 antibody, ETV-3, Ets variant gene 3, ETV 3, ETV 3 antibody
Specificity Human ETV3
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen ETV3 antibody was raised in mouse using recombinant Ets Variant Gene 3

Images

Immunofluorescent staining using ETV3 antibody (10R-1245)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ETV3 antibody.

Specifications

Host Mouse
Clone ETV3F4D10
Isotype IgG1
Method of Purification ETV3 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer ETV3 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Transcriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity{EC1}.

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Images

  • Immunofluorescent staining using ETV3 antibody (10R-1245) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ETV3 antibody.
  • Western Blot analysis of immunized recombinant protein using ETV3 antibody (10R-1245)

Availability: In stock

Price: $190.00
Size: 50 ug
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