FARS2 protein (His tag) (80R-1772)
Purified recombinant Human FARS2 protein
|Synonyms||FARS 2, FARS1 protein, PheRS protein, mitochondrial protein, FARS2, FARS 2 protein, HSPC320 protein, dJ520B18.2 protein, FARS-2 protein, FARS-2, Phenylalanyl-tRNA synthetase 2 protein|
Coomassie Blue stained SDS-PAGE of FARS2 protein (His tag) (80R-1772)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||37-451 amino acids: MGSSHHHHHH SSGLVPRGSH MPAAECATQR APGSVVELLG KSYPQDDHSN LTRKVLTRVG RNLHNQQHHP LWLIKERVKE HFYKQYVGRF GTPLFSVYDN LSPVVTTWQN FDSLLIPADH PSRKKGDNYY LNRTHMLRAH TSAHQWDLLH AGLDAFLVVG DVYRRDQIDS QHYPIFHQLE AVRLFSKHEL FAGIKDGESL QLFEQSSRSA HKQETHTMEA VKLVEFDLKQ TLTRLMAHLF GDELEIRWVD CYFPFTHPSF EMEINFHGEW LEVLGCGVME QQLVNSAGAQ DRIGWAFGLG LERLAMILYD IPDIRLFWCE DERFLKQFCV SNINQKVKFQ PLSKYPAVIN DISFWLPSEN YAENDFYDLV RTIGGDLVEK VDLIDKFVHP KTHKTSHCYR ITYRHMERTL SQREVRHIHQ ALQEAAVQLL GVEGRF|
|Purity||> 90% pure|
|Molecular Weight||50.6 kDa (436aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 30% glycerol, 1mM EDTA and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Phenylalanyl-tRNA synthetase 2, mitochondrial (PheRS), also known as FARS2, belongs to the class II aminoacyl- tRNA synthetase family. FARS2 is a mitochondrial matrix protein. Functioning as a monomer, FARS2 catalyzes the ATP-dependent conversion of L-phenylalanine and tRNA (Phe) to L-phenlalanyltRNA (Phe), an event that is crucial for proper translation and protein expression. Recombinant human FARS2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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