FasL protein (30R-AF033)

Purified soluble Human FasL protein

Synonyms Apo I Ligand protein, CD95L protein, TNFSF6 protein, soluble Fas Ligand protein, APTL protein, sFASL protein
Species Human
Protein Type Recombinant
Applications User optimized

Specifications

Residues Region of FasL protein corresponding to amino acids HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL.
Expression System CHO cells
Grade & Purity > 95% pure
Form & Buffer Supplied as a lyophilized powder.

Usage & Assay Information

Bioactivity Determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is approximately 10.0 ng/ml, corresponding to a specific activity of approximately 1 x 10^5 units/mg.

Storage & Safety

Storage Store at -20 deg C until reconstitution. Following reconstitution product may be stored at 4 deg C in the short term. For long term storage aliquot and freeze at -20 deg C. Avoid repeated freeze/thaw cycles.
Endotoxin Levels Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

General Information

Biological Significance Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain.

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Availability: In stock

Price: $225.00
Size: 10 ug
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