FBXO18 antibody (10R-1565)

Mouse monoclonal FBXO18 antibody

Synonyms Monoclonal FBXO18 antibody, Anti-FBXO18 antibody, MGC141935 antibody, FLJ14590 antibody, FBXO 18, FBH1 antibody, MGC141937 antibody, FBXO18, FBXO-18 antibody, F-box protein helicase 18 antibody, FBXO-18, Fbx18 antibody, FBXO 18 antibody, MGC131916 antibody
Specificity Human FBXO18
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen FBXO18 antibody was raised in mouse using recombinant Human F-Box Protein, Helicase, 18 (Fbxo18)

Images

Immunofluorescent staining using FBXO18 antibody (10R-1565)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-FBXO18 antibody.

Specifications

Host Mouse
Clone 2353C1a
Isotype IgG1
Method of Purification FBXO18 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer FBXO18 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different proteinprotein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbx class. It contains an F-box motif and seven conserved helicase motifs, and has both DNA-dependent ATPase and DNA unwinding activities. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.

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Images

  • Immunofluorescent staining using FBXO18 antibody (10R-1565) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-FBXO18 antibody.
  • Western Blot analysis of immunized recombinant protein using FBXO18 antibody (10R-1565)

Availability: In stock

Price: $190.00
Size: 50 ug
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