FGF1 protein (His tag) (80R-1948)

Recombinant mouse FGF1 protein

Synonyms FGF1, FGF-1, FGF-1 protein, Fam protein, Dffrx protein, FGF 1, Fgfa protein, Fibroblast growth factor 1 (acidic) protein, FGF 1 protein, Fgf-1 protein
Species Mouse
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of FGF1 protein (His tag) (80R-1948)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 16-155 amino acids: MGSSHHHHHH SSGLVPRGSH MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
Expression System E.coli
Purity > 90% pure
Molecular Weight 18 kDa (161aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations FGF1 protein (His tag) has been used in SDS PAGE, and may be suitable for use in other assays, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.
Endotoxin Levels < 1.0 EU per 1 microgram of protein (determined by LAL method)

General Information

Biological Significance Fibroblast growth factor 1, also known as FGF1, belongs to the fibroblast growth factor family. It is a potent mitogenic agent. FGF1 has been detected in large amounts in the brain. Other cells known to express FGF1 include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. The growth factor acts through activation of specific cell-surface receptors leading to intracellular tyrosine phosphorylation cascade. Recombinant mouse FGF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of FGF1 protein (His tag) (80R-1948) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $295.00
Size: 100 ug
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