Fibronectin antibody (biotin) (60R-FR003bt)

Rabbit polyclonal Fibronectin antibody (biotin) conjugated

Synonyms Polyclonal Fibronectin antibody, Anti-Fibronectin antibody, LETS antibody, CIG antibody, Cold insoluble globulin antibody, Transformation sensitive protein antibody, Migration stimulating factor antibody, MSF antibody
Specificity Human fibronectin
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, IP, WB
Immunogen Fibronectin antibody was raised in rabbit using fibronectin purified from human plasma as the immunogen.

Images

Immunohistochemical staining using Fibronectin antibody (biotin) (60R-FR003bt)

Immunohistochemistry with Fibronectin antibody biotin conjugated at 20X with negative controls (right). Tissue: kidney. Fixation: FFPE buffered formalin 10% conc. Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker (top) or EDTA pH 9.5 Pressure Cooker (bottom). 2ug/ml for 1 hour @ room T. Streptavidin Conjugated HRP 10 ug/ml circa 45 min at room temp. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Specifications

Host Rabbit
Method of Purification Fibronectin antibody (biotin) was purified by affinity chromatography.
Tag/Conjugate Biotin
Form & Buffer Liquid (sterile filtered) in 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 10 mg/ml BSA and 0.01% NaN3

Usage & Assay Information

Usage Recommendations ELISA: 1:5,000-1:20,000, IHC: 1:50-1:200, IP: 1:100, WB: 1:500-1:5,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Fibronectin is a high-molecular weight (~440 kDa) extracellular matrix glycoprotein that binds to membrane-spanning receptor proteins called integrins. In addition to integrins, fibronectin also binds extracellular matrix components such as collagen, fibrin and heparan sulfate proteoglycans (e.g. syndecans).

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Images

  • Immunohistochemical staining using Fibronectin antibody (biotin) (60R-FR003bt) | Immunohistochemistry with Fibronectin antibody biotin conjugated at 20X with negative controls (right). Tissue: kidney. Fixation: FFPE buffered formalin 10% conc. Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker (top) or EDTA pH 9.5 Pressure Cooker (bottom). 2ug/ml for 1 hour @ room T. Streptavidin Conjugated HRP 10 ug/ml circa 45 min at room temp. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
  • Immunohistochemical staining using Fibronectin antibody (biotin) (60R-FR003bt) | Tissue: human kidney at pH9 at 20x and 40x. Fixation: formalin fixed paraffin embedded. Fibronectin antibody at 10 ug/mL for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibronectin is cytoplasmic. Staining: Fibronectin as precipitated brown signal (A) with purple nuclear counterstain. With corresponding negative control (B).
  • Immunohistochemical staining using Fibronectin antibody (biotin) (60R-FR003bt) | Tissue: human kidney at pH6 at 20x and 40x. Fixation: formalin fixed paraffin embedded. Fibronectin antibody at 10 ug/mL for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibronectin is cytoplasmic. Staining: Fibronectin as precipitated brown signal (A) with purple nuclear counterstain. With corresponding negative control (B).

Availability: In stock

Price: $495.00
Size: 100 ug
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