FKBP3 antibody (10R-1372)

Mouse monoclonal FKBP3 antibody

Synonyms Monoclonal FKBP3 antibody, Anti-FKBP3 antibody, PPIase antibody, FKBP 3 antibody, FKBP-25 antibody, FKBP3, FK506-binding protein 3 antibody, FKBP 3, FKBP-3 antibody, FKBP-3
Specificity Human FKBP3
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen FKBP3 antibody was raised in mouse using recombinant Human Fk506 Binding Protein 3, 25Kda (Fkbp3)

Images

Immunofluorescent staining using FKBP3 antibody (10R-1372)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-FKBP3 antibody.

Specifications

Host Mouse
Clone 2364C3
Isotype IgG1
Method of Purification FKBP3 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer FKBP3 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It has a higher affinity for rapamycin than for FK506 and thus may be an important target molecule for immunosuppression by rapamycin.

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Images

  • Immunofluorescent staining using FKBP3 antibody (10R-1372) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-FKBP3 antibody.
  • Western Blot analysis of immunized recombinant protein using FKBP3 antibody (10R-1372) | Detection of FBP3 by Western Blot. Samples: Whole cell lysate from human HeLa (H, 50ug), mouse NIH3T3 (M, 50ug) and rat F2408 (R, 50ug) cells.

Availability: In stock

Price: $190.00
Size: 50 ug
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