FKBP6 protein (His tag) (80R-1324)

Purified recombinant Human FKBP6 protein

Synonyms FKBP-6, FK506 binding protein 6 protein, FKBP6, 36kDa 36 kDa FK506 binding protein protein, PPIase FKBP6 protein, FKBP36 protein, EC 5.2.1.8 protein, Immunophilin FKBP36 protein, Peptidyl prolyl cis trans isomerase protein, FKBP 6, FKBP-6 protein, Rotamase., FK506 binding protein 6 protein, FKBP 6 protein, PPIase protein, FKBP 36 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare assay buffer into a suitable container and pre-chilling on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant FKBP6 protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes.

Images

Coomassie Blue stained SDS-PAGE of FKBP6 protein (His tag) (80R-1324)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-327 amino acids: MGSSHHHHHH SSGLVPRGSH MGGSALNQGV LEGDDAPGQS LYERLSQRML DISGDRGVLK DVIREGAGDL VAPDASVLVK YSGYLEHMDR PFDSNYFRKT PRLMKLGEDI TLWGMELGLL SMRRGELARF LFKPNYAYGT LGCPPLIPPN TTVLFEIELL DFLDCAESDK FCALSAEQQD QFPLQKVLKV AATEREFGNY LFRQNRFYDA KVRYKRALLL LRRRSAPPEE QHLVEAAKLP VLLNLSFTYL KLDRPTIALC YGEQALIIDQ KNAKALFRCG QACLLLTEYQ KARDFLVRAQ KEQPFNHDIN NELKKLASCY RDYVDKEKEM WHRMFAPCGD GSTAGES
Expression System E.coli
Purity > 95% pure
Molecular Weight 39.3 kDa (347aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 40% glycerol, 1mM EDTA and 50mM NaCl.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity Specific activity is > 50 nM/min/ug, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1C in Tris-Hcl pH8.0 using chymotrypsin.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance FK506 binding protein 6, also known as FKBP6, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. Ubiquitously expressed in all tissues, FKBP6 is present at highest levels in testis, liver, kidney, skeletal muscle and heart. Deletion of FKBP6 may contribute to hypercalcemia and growth delay in Williams-Beuren syndrome. Recombinant human FKBP6, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Coomassie Blue stained SDS-PAGE of FKBP6 protein (His tag) (80R-1324) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 50 ug
OR
Shipping
View Our Distributors