FLAG antibody (10R-2139)
Mouse monoclonal FLAG antibody
|Synonyms||Monoclonal FLAG antibody, Anti-FLAG antibody, FLAG-tag antibody|
|Immunogen||FLAG antibody was raised in Mouse using synthetic peptide corresponding to aa(DYKDDDDK), conjugated to KLH as the immunogen.|
Western Blot analysis using FLAG antibody (10R-2139)
Western Blot showing Flag antibody used against two different fusion protein (1), (2) with flag tag.
|Form & Buffer||Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:10,000, WB: 1:500-1:2000|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||The FLAG antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.|
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