FLAG antibody (10R-2139)

Mouse monoclonal FLAG antibody

Synonyms Monoclonal FLAG antibody, Anti-FLAG antibody, FLAG-tag antibody
Applications ELISA, WB
Immunogen FLAG antibody was raised in Mouse using synthetic peptide corresponding to aa(DYKDDDDK), conjugated to KLH as the immunogen.

Images

Western Blot analysis using FLAG antibody (10R-2139)

Western Blot showing Flag antibody used against two different fusion protein (1), (2) with flag tag.

Specifications

Host Mouse
Clone 2F11G1
Isotype IgG2b
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The FLAG antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.

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Images

  • Western Blot analysis using FLAG antibody (10R-2139) | Western Blot showing Flag antibody used against two different fusion protein (1), (2) with flag tag.

Availability: In stock

Price: $295.00
Size: 100 ul
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