FOS antibody (10R-1389)

Mouse monoclonal FOS antibody

Synonyms Monoclonal FOS antibody, Anti-FOS antibody, c-fos antibody, FBJ murine osteosarcoma viral oncogene homolog antibody
Specificity Human FOS
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen FOS antibody was raised in mouse using recombinant Human V-Fos Fbj Murine Osteosarcoma Viral Oncogene Homolog

Images

Immunofluorescent staining using FOS antibody (10R-1389)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-FOS antibody.

Specifications

Host Mouse
Clone 554C1a
Isotype IgG1
Method of Purification FOS antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer FOS antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.

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Images

  • Immunofluorescent staining using FOS antibody (10R-1389) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-FOS antibody.
  • Western Blot analysis of immunized recombinant protein using FOS antibody (10R-1389)

Availability: In stock

Price: $190.00
Size: 50 ug
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