GABPA antibody (10R-1981)

Mouse monoclonal GABPA antibody

Synonyms Monoclonal GABPA antibody, Anti-GABPA antibody, alpha subunit antibody, NFT2 antibody, NRF2A antibody, E4TF1A antibody, E4TF1-60 antibody, NRF2 antibody, GA binding protein transcription factor
Cross Reactivity Human, Mouse
Applications ELISA, IF, WB
Immunogen GABPA antibody was raised in Mouse using a purified recombinant fragment of human GABPA (aa120-190) expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using GABPA antibody (10R-1981) (GABPA antibody)

Confocal immunofluorescence analysis of Hela cells using GABPA antibody (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Specifications

Host Mouse
Clone 8C1B10
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance GABPA is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, it may play a role in the Down Syndrome phenotype.

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Images

  • Immunofluorescent staining using Western Blot analysis using GABPA antibody (10R-1981) (GABPA antibody) | Confocal immunofluorescence analysis of Hela cells using GABPA antibody (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Western Blot analysis using GABPA antibody (10R-1981) | Western Blot showing GABPA antibody used against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GABPA cDNA (2).
  • Western Blot analysis using GABPA antibody (20227) | Western Blot showing using GABPA antibody used against Hela (1), A549 (2), MCF-7 (3), NIH/3T3 (4) and SMMC-7721 (5) cell lysate.

Availability: In stock

Price: $345.00
Size: 100 ul
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