GALK1 protein (His tag) (80R-1403)
Purified recombinant Human GALK1 protein
Overview
Overview
| Synonyms | GALK protein, GALK 1 protein, GK1 protein, GALK 1, GALK-1, Galactokinase, GALK1, GALK-1 protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of GALK1 protein (His tag) (80R-1403)
Figure annotation denotes ug of protein loaded and % gel used.
GALK1 protein (His tag)
Coomassie Blue stained SDS-PAGE of GALK1 protein (His tag) (80R-1403)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-392 amino acids: MGSSHHHHHH SSGLVPRGSH MAALRQPQVA ELLAEARRAF REEFGAEPEL AVSAPGRVNL IGEHTDYNQG LVLPMALELM TVLVGSPRKD GLVSLLTTSE GADEPQRLQF PLPTAQRSLE PGTPRWANYV KGVIQYYPAA PLPGFSAVVV SSVPLGGGLS SSASLEVATY TFLQQLCPDS GTIAARAQVC QQAEHSFAGM PCGIMDQFIS LMGQKGHALL IDCRSLETSL VPLSDPKLAV LITNSNVRHS LASSEYPVRR RQCEEVARAL GKESLREVQL EELEAARDLV SKEGFRRARH VVGEIRRTAQ AAAALRRGDY RAFGRLMVES HRSLRDDYEV SCPELDQLVE AALAVPGVYG SRMTGGGFGG CTVTLLEASA APHAMRHIQE HYGGTATFYL SQAADGAKVL CL |
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| Expression System | E.coli |
| Purity | > 95% pure |
| Molecular Weight | 44.4 (412aa) confirmed by MALDI-TOF |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT. |
| Concentration | 0.5 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | GALK1, also often designated galactokinase, is important in the first step of the galactose metabolism pathway. (ATP + D-galactose = ADP + alpha-D-galactose 1-phosphate) Defects in GALK1 are the cause of galactosemia II. It is an autosomal recessive deficiency characterized by congenital cataracts during infancy and presenile cataracts in the adult population. The cataracts are secondary to accumulation of galactitol in the lenses. Recombinant human GALK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
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