GAPDH antibody (10R-1931)

Mouse monoclonal GAPDH antibody

Synonyms Monoclonal GAPDH antibody, Anti-GAPDH antibody, Glyceraldehyde-3-phosphate dehydrogenase antibody
Cross Reactivity Human
Applications ELISA, IF, IHC, WB
Immunogen GAPDH antibody was raised in Mouse using a purified recombinant fragment of human GAPDH expressed in E. coli as the immunogen.

Images

Western Blot analysis using GAPDH antibody (10R-1931)

Western Blot showing GAPDH antibody used against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate.

Specifications

Host Mouse
Clone 1A10
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling.

References
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Kelly G. Bryanta, Jeanette Camachoc, Jean-François Jasmina, Chenguang Wanga, Sankar Addyad, Mathew C. Casimirod, Paolo Fortinad, Sucharitha Balasubramaniamd, Karen E. Knudsend, Roland Schwartingc, Michael P. Lisantia, Isabelle Mercier

Caveolin-1 overexpression enhances androgen-dependent growth and proliferation in the mouse prostate

The International Journal of Biochemistry & Cell Biology

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Casey D. Wright, Steven C. Wu, Erika F. Dahl, Alan J. Sazama, Timothy D. O'Connell

Nuclear localization drives α1-adrenergic receptor oligomerization and signaling in cardiac myocytes

Cellular Signalling

Volume: 24 Issue: 3 Page: 794-802 DOI: 10.1016/j.cellsig.2011.11.014

Etsushi Kuroda, Ken J. Ishii, Satoshi Uematsu, Keiichi Ohata, Cevayir Coban, Shizuo Akira, Kosuke Aritake, Yoshihiro Urade, Yasuo Morimoto

Silica Crystals and Aluminum Salts Regulate the Production of Prostaglandin in Macrophages via NALP3 Inflammasome-Independent Mechanisms

Immunity

Volume: 34 Issue: 4 Page: 514-526 DOI: 10.1016/j.immuni.2011.03.019

Daniel Adessea, Luciana R. Garzonia, Huan Huangc, Herbert B. Tanowitzc, Maria de Nazareth Meirellesa, David C. Spray

Trypanosoma cruzi induces changes in cardiac connexin 43 expression

Microbes and Infection

Volume: 10 Issue: 1 Page: 21-28 DOI: 10.1016/j.micinf.2007.09.017

Shinong Wang, Raimund Hirschberg

Diabetes-relevant regulation of cultured blood outgrowth endothelial cells

Microvascular Research

Volume: 78 Issue: 2 Page: 174-179 DOI: 10.1016/j.mvr.2009.06.002

Jason Aligo, Shuaizheng Jia, David Manna, Kouacou V. Konan

Formation and function of hepatitis C virus replication complexes require residues in the carboxy-terminal domain of NS4B protein

Virology

Volume: 393 Issue: 1 Page: 68-83 DOI: 10.1016/j.virol.2009.07.033

Joerg Heinekea, b, Kai C. Wollertb, Hanna Osinskaa, Michelle A. Sargenta, Allen J. Yorka, Jeffrey Robbinsa, Jeffery D. Molkentin

Calcineurin protects the heart in a murine model of dilated cardiomyopathy

Journal of Molecular and Cellular Cardiology

Volume: 48 Issue: 6 Page: 1080-1087 DOI: 10.1016/j.yjmcc.2009.10.012

Jianjian Shia, Yi-Wei Zhanga, Yu Yanga, Lumin Zhanga, Lei Wei

ROCK1 plays an essential role in the transition from cardiac hypertrophy to failure in mice

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Volume: 49 Issue: 5 Page: 819-828 DOI: 10.1016/j.yjmcc.2010.08.008

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Images

  • Western Blot analysis using GAPDH antibody (10R-1931) | Western Blot showing GAPDH antibody used against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate.
  • Immunohistochemical staining using GAPDH antibody (10R-1931) | Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH antibody with DAB staining.

Availability: In stock

Price: $225.00
Size: 100 ul
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