GAPDH antibody (10R-1931)

Mouse monoclonal GAPDH antibody

Synonyms Monoclonal GAPDH antibody, Anti-GAPDH antibody, Glyceraldehyde-3-phosphate dehydrogenase antibody
Cross Reactivity Human
Applications ELISA, IF, IHC, WB
Immunogen GAPDH antibody was raised in Mouse using a purified recombinant fragment of human GAPDH expressed in E. coli as the immunogen.


Western Blot analysis using GAPDH antibody (10R-1931)

Western Blot showing GAPDH antibody used against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate.


Host Mouse
Clone 1A10
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling.

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  • Western Blot analysis using GAPDH antibody (10R-1931) | Western Blot showing GAPDH antibody used against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate.
  • Immunohistochemical staining using GAPDH antibody (10R-1931) | Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH antibody with DAB staining.

Availability: In stock

Price: $225.00
Size: 100 ul
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