GAPDH antibody (10R-8399)

Mouse monoclonal GAPDH antibody

Synonyms Monoclonal GAPDH antibody, Anti-GAPDH antibody, GAPDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, 38 kDa BFA-dependent ADP-ribosylation substrate antibody, aging-associated gene 9 protein antibody, BARS-38 antibody, bb02e05 antibody, BOS_5957 antibody, CDABP0047 antibody, g3pd antibody, G3PDH antibody, gapd antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, glyceraldehyde phosphate dehydrogenase antibody, glyceraldhyde 3-phosphate dehydrogenase antibody, glycerladehyde 3 phosphate dehydrogenase antibody, KNC-NDS6 antibody, Peptidyl-cysteine S-nitrosylase GAPDH antibody
Cross Reactivity Human
Applications ELISA, IP, WB
Immunogen GAPDH antibody was raised in Mouse using a recombinant human protein purified from E.coli as the immunogen

Images

Western Blot analysis using GAPDH antibody (10R-8399)

Specifications

Host Mouse
Clone 8C2
Isotype IgG1 kappa
Method of Purification GAPDH antibody was purified by ammonium sulfate precipitation
Form & Buffer Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol

Usage & Assay Information

Usage Recommendations WB: 1:10000, IP: 1-2 ul

Storage & Safety

Storage Aliquot and store for 1 year at -20 deg C, avoid freeze/thaw cycles

General Information

Biological Significance Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer of identical 37 kDa subunits. GAPDH catalyzes the reversible reduction of 1,3-bisphos-phoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, this ubiquitously expressed enzyme also displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Other studies also provide evidence of GAPDH playing an essential part of the program of gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases.

References
Add a Paper

Wardit Tigchelaar , Hongjuan Yu , Anne Margreet De Jong , Wiek H van Gilst , Pim van der Harst , B Daan Westenbrink , Rudolf A de Boer , Herman H.W. Sillje

Loss of mitochondrial exo/endonuclease EXOG affects mitochondrial respiration and induces ROS mediated cardiomyocyte hypertrophy

American Journal of Physiology

PMID: 25377088 DOI: 10.1152/ajpcell.00227.2014

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Images

  • Western Blot analysis using GAPDH antibody (10R-8399) |

Availability: In stock

Price: $295.00
Size: 100 ul
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