GATAD1 antibody (10R-1253)

Mouse monoclonal GATAD1 antibody

Synonyms Monoclonal GATAD1 antibody, Anti-GATAD1 antibody, GATAD1, GATA Zinc finger domain containing 1antibody, FLJ22489 antibody, GATAD 1, GATAD 1 antibody, RG083M05.2 antibody, ODAG antibody, GATAD-1, GATAD-1 antibody
Specificity Human GATAD1
Cross Reactivity Human, Mouse. Other species not studied
Applications Dot Blot, FC, ICC, WB
Immunogen GATAD1 antibody was raised in mouse using recombinant Gata Zinc Finger Domain Containing 1 (Gatad1)

Images

Flow Cytometric assay using GATAD1 antibody (10R-1253)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-GATAD1 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone GATA9A1D3
Isotype IgG2b
Method of Purification GATAD1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer GATAD1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, FC: 0.5-2 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene is associated with ocular development.

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Images

  • Flow Cytometric assay using GATAD1 antibody (10R-1253) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-GATAD1 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using GATAD1 antibody (10R-1253) | Detection of GATAD1 by Western Blot. Samples: Whole cell lysate from human A2058 (H, 50ug) and mouse NIH3T3 (M, 50ug) cells.
  • Immunofluorescent staining using GATAD1 antibody (10R-1253) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-GATAD1 antibody.

Availability: In stock

Price: $190.00
Size: 50 ug
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