GCAT protein (His tag) (80R-2787)

Purified recombinant GCAT protein (His tag)

Synonyms Glycine C-acetyltransferase protein, 2-amino-3-ketobutyrate coenzyme A ligase protein, mitochondrial protein, AKB ligase protein, Aminoacetone synthase protein, Glycine acetyltransferase protein, KBL protein, GCAT
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of GCAT protein (His tag) (80R-2787)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 22-419 aa: MGSSHHHHHH SSGLVPRGSH MSALAQLRGI LEGELEGIRG AGTWKSERVI TSRQGPHIRV DGVSGGILNF CANNYLGLSS HPEVIQAGLQ ALEEFGAGLS SVRFICGTQS IHKNLEAKIA RFHQREDAIL YPSCYDANAG LFEALLTPED AVLSDELNHA SIIDGIRLCK AHKYRYRHLD MADLEAKLQE AQKHRLRLVA TDGAFSMDGD IAPLQEICCL ASRYGALVFM DECHATGFLG PTGRGTDELL GVMDQVTIIN STLGKALGGA SGGYTTGPGP LVSLLRQRAR PYLFSNSLPP AVVGCASKAL DLLMGSNTIV QSMAAKTQRF RSKMEAAGFT ISGASHPICP VMLGDARLAS RMADDMLKRG IFVIGFSYPV VPKGKARIRV QISAVHSEED IDRCVEAFVE VGRLHGALP
Expression System E.coli
Purity > 85% pure
Molecular Weight 45.0 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Concentration 1 mg/ml

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Recombinant human GCAT protein, fused to His-tag at N-terminus, was expressed in E.coli.

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Images

  • SDS-PAGE analysis of GCAT protein (His tag) (80R-2787) | Figure annotation denotes ug of protein loaded and % gel used.
  • SDS-PAGE analysis of GCAT protein (His tag) (80R-2787) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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