GCDH antibody (70R-1103)

Rabbit polyclonal GCDH antibody raised against the C terminal of GCDH

Synonyms Polyclonal GCDH antibody, Anti-GCDH antibody, Glutaryl-Coenzyme A Dehydrogenase antibody, ACAD5 antibody, GCD antibody
Specificity GCDH antibody was raised against the C terminal of GCDH
Cross Reactivity Human, Mouse, Rat, Dog, ZebraFish
Applications IHC, WB
Immunogen GCDH antibody was raised using the C terminal of GCDH corresponding to a region with amino acids IARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTHDIHALILGRAITG
Assay Information GCDH Blocking Peptide, catalog no. 33R-3897, is also available for use as a blocking control in assays to test for specificity of this GCDH antibody

Images

Western Blot analysis using GCDH antibody (70R-1103)

GCDH antibody (70R-1103) used at 2.5 ug/ml to detect target protein.

Specifications

Host Rabbit
Method of Purification Total IgG Protein A purified
Molecular Weight 48 kDa (MW of target protein)
Form & Buffer Lyophilized powder. Add 100ul distilled water for a 1mg/ml concentration of GCDH antibody in PBS
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 2.5 ug/ml; IHC: 4-8 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for short periods. For longer periods of storage, store at -20 deg C. Avoid repeat freeze-thaw cycles.

General Information

Biological Significance GCDH belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45 kDa subunits.

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Images

  • Western Blot analysis using GCDH antibody (70R-1103) | GCDH antibody (70R-1103) used at 2.5 ug/ml to detect target protein.
  • Immunohistochemical staining using GCDH antibody (70R-1103) | GCDH antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Skeletal muscle cells (arrows) in Human Muscle. Magnification is at 400X

Availability: In stock

Price: $275.00
Size: 100 ug
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