GCLM antibody (10R-7933)

Mouse monoclonal GCLM antibody

Synonyms Monoclonal GCLM antibody, Anti-GCLM antibody, Glutamate-cysteine ligase antibody, GLCLR antibody, GCLM
Cross Reactivity Human
Applications ELISA, WB
Immunogen GCLM antibody was purified by recombinant human GCLM (1-274aa) purified from E. coli

Images

Western Blot analysis using GCLM antibody (10R-7933)

HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT2D12
Isotype IgG1 kappa
Method of Purification GCLM antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied in liquid form in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:3000

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance GCLM (Glutamate-cysteine ligase, modifier subunit), also known as gamma-glutamylcysteine synthetase, is first rate limiting enzyme of glutathione synthesis. GCLM is heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. GCLM deficiency has been implicated in some forms of hemolytic anemia. Also, GCLM is a molecular target for amelioration of cisplatin restance in lung cancer.

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Images

  • Western Blot analysis using GCLM antibody (10R-7933) | HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Western Blot analysis using GCLM antibody (10R-7933) | HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: $165.00
Size: 50 ul
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