GFP antibody (10R-1145)

Mouse monoclonal GFP antibody

Synonyms Monoclonal GFP antibody, Anti-GFP antibody, Green fluorescent protein antibody
Specificity Human
Applications ELISA, WB
Immunogen GFP antibody was raised in mouse using recombinant GFP (1-238aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of GFP antibody (10R-1145)

The recombinant protein GFP (20, 40 and 80ng) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-GFP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : GFP 20ng Lane 2 : GFP 40ng Lane 3 : GFP 80ng

Specifications

Host Mouse
Clone AT2G5
Isotype IgG2a kappa
Source Anti-GFP antibody, clone AT2G5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant GFP protein.
Method of Purification GFP antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:1,000-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

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Images

  • Western Blot analysis of GFP antibody (10R-1145) | The recombinant protein GFP (20, 40 and 80ng) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-GFP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : GFP 20ng Lane 2 : GFP 40ng Lane 3 : GFP 80ng

Availability: In stock

Price: $265.00
Size: 100 ul
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