GFP antibody (10R-8423)

Mouse monoclonal GFP antibody

Synonyms Monoclonal GFP antibody, Anti-GFP antibody, GFP Green Fluorescent Protein antibody
Applications ELISA, IP
Immunogen GFP antibody was raised in Mouse using Recombinant green fluorescent protein as the immunogen

Images

Western Blot analysis using GFP antibody (10R-8423)

Specifications

Host Mouse
Clone 18A11
Isotype IgG1 kappa
Method of Purification GFP antibody was purified by ammonium sulfate precipitation
Form & Buffer Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol

Usage & Assay Information

Usage Recommendations IP: 1 ul

Storage & Safety

Storage Aliquot and store for 1 year at -20 deg C, avoid freeze/thaw cycles

General Information

Biological Significance Green fluorescent protein (GFP) isolated from jellyfish Aequorea aequorea is a 238 amino acid protein with an apparent molecular weight of about 27-30 kDa on SDS-PAGE. Its chromophore is formed by cyclisation and oxidation of the three amino acids Ser65, Tyr66, and Gly67. The numerous applications include : using GFP as a reporter for gene expression, as a marker to study cell lineage during development and as a tag to localize proteins in living cells. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology.

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Images

  • Western Blot analysis using GFP antibody (10R-8423) |

Availability: In stock

Price: $295.00
Size: 100 ul
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