GFP antibody (20R-GR011)

Rabbit polyclonal GFP antibody

Synonyms Polyclonal GFP antibody, Anti-GFP antibody, Green Fluorescent Protein antibody
Cross Reactivity This antibody reacts with wild -type GFP and it?s variants, such as EGFP, EBFP
Applications IP, WB
Immunogen GFP antibody was raised in rabbit using E. Coli-expressed full length green fluorescent protein as the immunogen.

Specifications

Host Rabbit
Form & Buffer Supplied as a lyophilized Potein A purified rabbit IgG with 0.1% NaN3. Reconstitue in distilled water.

Usage & Assay Information

Usage Recommendations IP: 1:200-1:500, WB: 1:5,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.

References
Add a Paper

Denise Orozco, Sabina Tahirovic, Kristin Rentzsch, BenjaminM. Schwenk, Christian Haass

Loss of fused in sarcoma (FUS) promotes pathological Tau splicing

EMBO Reports

Volume: 13 Issue: 2012 Page: 759-764 PMID: 22710833 DOI: 10.1038/embor.2012.90

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Availability: In stock

Price: $484.00
Size: 200 ug
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