GFP antibody (HRP) (60R-GG002hrp)

Goat polyclonal GFP antibody (HRP) conjugated

Synonyms Polyclonal GFP antibody, Anti-GFP antibody, Green Fluorescent Protein Antibody, GFP conjugate
Specificity Jellyfish GFP
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen GFP antibody (HRP) was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria as the immunogen.

Images

Western blot analysis using GFP antibody (HRP) (60R-GG002hrp)

Western Blot of GFP antibody (HRP). Lane 1: 50ng of GFP. Lane 2: none. none. GFP antibody (HRP) secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS (diluted to 1X) 30 min at 20 deg C. Size: 28 kDa for GFP.

Specifications

Host Goat
Method of Purification GFP antibody (HRP) was purified by affinity chromatography.
Tag/Conjugate HRP
Form & Buffer Lyophilized from 0.02M K2PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% gentamycin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000 - 1:5,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

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Images

  • Western blot analysis using GFP antibody (HRP) (60R-GG002hrp) | Western Blot of GFP antibody (HRP). Lane 1: 50ng of GFP. Lane 2: none. none. GFP antibody (HRP) secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS (diluted to 1X) 30 min at 20 deg C. Size: 28 kDa for GFP.

Availability: In stock

Price: $695.00
Size: 1 mg
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